Researchers have found a new role for the transcription factor (proteins that regulate the transcription, or copying, of genes). In the fruit fly, this transcription factor, named Traffic Jam, ...

Boston)—The global birthrate has been in significant decline for decades. In the U.S., couples are deciding to have children later in life. A 2022 ...

Through a combination of chromatin immunoprecipitation, transcriptional reporter assays, subcellular localization analyses, and protein aggregation experiments, the authors provide solid evidence that ...

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DNA immunoprecipitation-sequencing (DIP-seq). 5-methylcytosine (5mC). 5-hydroxymethylcytosine (5hmC). 5-formylcytosine (5fC). 5-carboxylcytosine (5caC). Nucleosome positioning and Open Chromatin ...

The CUT&Tag-IT R-loop Assay Kit is designed to be faster and more efficient than DNA:RNA immunoprecipitation (DRIP) and chromatin immunoprecipitation (ChIP) methods, and is compatible with 200,000 to ...

Two or 10 million cells were harvested for RNA extraction and chromatin immunoprecipitation assays, respectively. 2.9 RNA extraction and quantitative RT-PCR The cells harvested (2 × 10 6) were washed ...

β-actin was used as an internal control. Chromatin immunoprecipitation (ChIP) assays (Millipore, 17-408) were carried out per the manufacturer’s instructions. Chromatin was sheared by sonication at ...

Design Transcriptional regulation was assessed by chromatin immunoprecipitation, promoter mutation and luciferase reporter assays. Hepatocellular proliferation and tumourigenesis were examined by ...