and expression testing of vectors featuring various affinity tags (i.e., His, GST, FLAG) and/or fusion partners (i.e., TRX, MBP, EGFP) in order to maximize the expression level of the target protein ...

As an agent, your first responsibility is to identify the league for which your particular client (protein) is best suited ... Beginning with this issue, we will take a look at expression vectors ...

The major core services are the following: Up-front consultation on experimental design and assistance with construction of various recombinant protein expression vectors. Expression of recombinant ...

To compare cell growth, protein expression level and final purified target yield, we also performed a standard induction with IPTG using the same vectors, host and fusion proteins. The crude ...

Protein-coding, expression-ready genes are available in vectors tagged for protein identification and purification. I think GenScript provides absolutely excellent products in a timely fashion.

Figure 1: GFP-fragment reassembly vectors and cloning linker sequences ... Avoid fusions to the GFP fragments at the site of protein-protein interaction and consider optimizing the spacer length ...

Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as ...

For example, immune responses against viruses limit these vectors’ repeated use, while the composition of LNPs directs them preferentially to the liver. “We thought if we used a fusion protein to ...

If the treatment is successful, the new gene will make a functional protein. (Illustration ... and non-dividing cells and provide high levels of gene expression. However, adenoviral vectors can ...