β-actin was used as an internal control. Chromatin immunoprecipitation (ChIP) assays (Millipore, 17-408) were carried out per the manufacturer’s instructions. Chromatin was sheared by sonication at ...

Researchers from Japan have developed a cell-based reporter assay for quantification of chemical ... or reversible changes to DNA and chromatin (packaged DNA-protein complexes) have been ...

The CUT&Tag-IT R-loop Assay Kit is designed to be faster and more efficient than DNA:RNA immunoprecipitation (DRIP) and chromatin immunoprecipitation (ChIP) methods, and is compatible with 200,000 to ...

Epigenetic regulation orchestrated by changes in chromatin openness modulates behavioral plasticity by controlling gene expression. However, the gene regulation mechanisms by which chromatin openness ...

Samples were sequenced to an average target depth of 450 million reads per library. For identification of chromatin loop interactions, we used the KR-corrected 5 kb and 10 kb bin-sized cooler files ...

Two or 10 million cells were harvested for RNA extraction and chromatin immunoprecipitation assays, respectively. 2.9 RNA extraction and quantitative RT-PCR The cells harvested (2 × 10 6) were washed ...

According to Gilmour, these initial experiments established the feasibility of protein-DNA crosslinks in intact cells combined with immunoprecipitation ... assay. In this first run of ChIP, they found ...

Chromatin immunoprecipitation (ChIP) assay was used to analyze NF-κB's transcription regulation of NF-κB on the Runx2 promoter. OST medium-treated VICs exhibited a greater expression of NF-κB, CREB, ...

Click here to download 'A solid phase chromatin immunoprecipitation assay (ChIP)' brochure The kits have been optimised for 1000 ng sample sizes, which allows for better results from smaller ...

Chromatin immunoprecipitation (ChIP) is a technique researchers employ to obtain a snapshot of protein-DNA interactions in the cell. ChIP provides a picture of where your protein of interest is in the ...